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Journal: Emerging Microbes & Infections
Article Title: A real-time and high-throughput neutralization test based on SARS-CoV-2 pseudovirus containing monomeric infrared fluorescent protein as reporter
doi: 10.1080/22221751.2021.1925163
Figure Lengend Snippet: Neutralization test based on SARS-CoV-2 pseudoviruses with miRFP reporter. (A,B) miRFP signals (top) and neutralization curves and NT 50 titers (bottom) to pseudoviruses Str and D614G at 72 h post-infection in HEK-293T-hACE2 cells of a convalescent-phase serum sample from a confirmed COVID-19 case (A) and neutralization curves and NT 50 titers of other 4 confirmed COVID-19 cases (B). Data are means and standard deviations of duplicates from one representative experiment of two. (C) Comparison between NT 50 titers to pseudoviruses Str and D614G for 15 confirmed COVID-19 cases. The two-tailed Wilcoxon rank signed test (GraphPad 6.0). (D) Relationship between NT 50 titers to pseudovirus Str and sampling days. (E-J) Relationship between NT 50 titers to pseudoviruses Str and PRNT 50 (E), PRNT 80 (F) or PRNT 90 (G) titers to SARS-CoV-2 USA-WA-1 strain (containing D614) and relationship between NT 50 titers to pseudoviruses D614G and PRNT 50 (H), PRNT 80 (I) or PRNT 90 (J) titers to SARS-CoV-2 USA-WA-1 strain. The two-tailed Spearman correlation test (GraphPad 6.0).
Article Snippet: The two-tailed
Techniques: Neutralization, Infection, Comparison, Two Tailed Test, Sampling